BioImager.Focus Class Reference

Public Member Functions
void	SetFocus (double f)
double	GetFocus ()
double	GetFocus (bool update)
PointD	GetSWLimit ()
void	SetSWLimit (double xd, double yd)

Static Public Attributes
static double	upperLimit
static double	lowerLimit

Detailed Description

Definition at line 244 of file Microscope.cs.

Constructor & Destructor Documentation

Focus()

BioImager.Focus.Focus

(

)

Definition at line 249 of file Microscope.cs.

        {
 
        }

Member Function Documentation

GetFocus() [1/2]

double BioImager.Focus.GetFocus

(

)

This function returns the z-axis position

Returns

The z-axis position of the microscope.

Definition at line 292 of file Microscope.cs.

        {
            return GetFocus(false);
        }

GetFocus() [2/2]

double BioImager.Focus.GetFocus

(

bool

update

)

If the microscope is a Prior, get the Z position from the SDK. If it's a MTB, get the Z position from the MTB SDK. If it's a PMicroscope, get the Z position from the PMicroscope SDK. If it's a custom microscope, get the Z position from the custom SDK

Parameters

update

boolean, if true, the focus is updated from the microscope

Returns

The z position of the microscope.

Definition at line 304 of file Microscope.cs.

        {
            if (Properties.Settings.Default.PMicroscope)
            {
                Point3D p;
                Microscope.pMicroscope.GetPosition3D(out p, update);
                return p.Z;
            }
            else if(Properties.Settings.Default.PycroManager)
            {
                Point3D p;
                MicroManager.GetPosition3D(out p, update);
                return p.Z;
            }
            if (Properties.Settings.Default.LibPath.Contains("MTB"))
            {
                BioImager.MicroscopeConsole.Command com = BioImager.MicroscopeConsole.RunCommand
                    (new BioImager.MicroscopeConsole.Command(BioImager.MicroscopeConsole.Command.Type.GetFocus, null));
                return com.doubles[0];
            }
            if (Properties.Settings.Default.LibPath.Contains("Prio"))
            {
                return Microscope.sdk.GetZ();
            }
            if(Automation.Properties.ContainsKey("GetFocus"))
            {
                return (double)Automation.GetProperty("GetFocus");
            }
            return z;
        }

GetSWLimit()

PointD BioImager.Focus.GetSWLimit

(

)

It gets the lower and upper limits of the focus axis

Returns

The return value is a PointD object.

Definition at line 338 of file Microscope.cs.

        {
            if (Properties.Settings.Default.LibPath.Contains("MTB"))
            {
                BioImager.MicroscopeConsole.Command com = BioImager.MicroscopeConsole.RunCommand(
                    new BioImager.MicroscopeConsole.Command(BioImager.MicroscopeConsole.Command.Type.GetStageSWLimit, null));
                upperLimit = com.doubles[0];
                lowerLimit = com.doubles[1];
                return new PointD(upperLimit,lowerLimit);
            }
            return new PointD(lowerLimit, upperLimit);
        }

SetFocus()

void BioImager.Focus.SetFocus

(

double

f

)

Sets the z-axis based on microscope configuration.

Parameters

f	the focus value

Definition at line 257 of file Microscope.cs.

        {
            if (Recorder.recordMicroscope)
                Recorder.AddLine("Microscope.Focus.SetFocus(" + f + ");");
            if (Properties.Settings.Default.PMicroscope)
            {
                PointD p = Microscope.Stage.GetPosition(true);
                Microscope.pMicroscope.SetPosition(new Point3D(p.X,p.Y,f));
            }
            else
            if (Properties.Settings.Default.PycroManager)
            {
                PointD p = Microscope.Stage.GetPosition(true);
                MicroManager.SetPosition(new Point3D(p.X, p.Y, f));
            }
            else
            if (Properties.Settings.Default.LibPath.Contains("MTB"))
            {
                BioImager.MicroscopeConsole.RunCommand(
                    new BioImager.MicroscopeConsole.Command(BioImager.MicroscopeConsole.Command.Type.SetFocus,new double[] { f}));
                z = f;
            }
            else if (Properties.Settings.Default.LibPath.Contains("Prio"))
            {
                Microscope.sdk.SetZ(f);
            }
            if (Automation.Properties.ContainsKey("SetFocus"))
            {
                Automation.SetProperty("SetFocus", f.ToString());
            }
        }

SetSWLimit()

void BioImager.Focus.SetSWLimit	(	double	xd,
		double	yd )

The function takes two double values, xd and yd, and sets the upper and lower limits of the focus axis

Parameters

xd	the upper limit of the focus
yd	the lower limit of the focus

Definition at line 356 of file Microscope.cs.

        {
            if (Properties.Settings.Default.LibPath.Contains("MTB"))
            {
                BioImager.MicroscopeConsole.RunCommand(
                   new BioImager.MicroscopeConsole.Command(BioImager.MicroscopeConsole.Command.Type.SetFocusSWLimit,
                   new double[] { xd, yd }));
            }
            upperLimit = xd;
            lowerLimit = yd;
            if (Recorder.recordMicroscope)
                Recorder.AddLine("Microscope.Focus.SetSWLimit(" + xd + "," + yd + ");");
        }

Member Data Documentation

lowerLimit

double BioImager.Focus.lowerLimit

static

Definition at line 247 of file Microscope.cs.

upperLimit

double BioImager.Focus.upperLimit

static

Definition at line 246 of file Microscope.cs.

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The documentation for this class was generated from the following file:

• BioImager/Source/Microscope.cs